DNA Siblingship Tests -- a Sample Case

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DNA Siblingship Tests — a Sample Case

The question was raised whether one of four siblings had a different father than the other three. Siblingship tests were run between A, the child in question, and her brothers, B and C. Then another test was run comparing A to her sister, D. Results between A and B showed that she was 27 times more likely to be his sister, than not. Results comparing A to C and D were deemed "inconclusive," which here means not certainly yes or no (i.e., the inconclusive results do not falsify the hypothesis of relationship). As there is no doubt that B, C, and D are full siblings, and there is no doubt that A and B are siblings, then logically, all four must be full siblings.

Each parent provides two copies of each marker; therefore, there can be no more than four different values for any one marker among the children. In no case, below, do the siblings collectively possess more than four different values at any one marker. The hypothesis of siblingship is supported (i.e., not falsified) by these results.

Protocol			Chromosome		Marker	A		B		C		D		Parental Values
SGM (n=6)	SGM+ (n=10+1)	CODIS (n=13+1)	#	Location	Marker	A		B		C		D		Parental Values
	X		2	q35-37.1	D2S1338	19	23	17	17	17	23	17	17	17, 17, 19, 23
		X	2	p23-2p645	TPOX	8	8	8	11	8	8	8	11	8, 8, 11
	X	X	3	p21	D3S1358	15	16	16	16	15	16	15	16	15, 16, 16
X	X	X	4	q28	FGA	23	24	20	21	23	24	23	24	20, 21, 23, 24
		X	5	q21-q31	D5S818	12	12	12	12	12	12	12	12	12, 12
		X	5	q33.3-34	CSF1P0	10	11	10	11	12	12	10	12	10, 11, 12, 12
		X	7	q	D7S820	10	11	10	11	10	11	10	12	10, 11, 12
X	X	X	8	q24.1-24.2	D8S1179	10	13	10	13	14	14	10	14	10, 13, 14
X	X	X	11	p15.5	TH01	6	6	6	9.3	6	9.3	6	6	6, 6, 9.3
X	X	X	12	p12-pter	VWA	16	17	14	15	14	15	15	17	14, 15, 16, 17
		X	13	q22-q31	D13S317	7	9	9	12	7	14	9	12	7, 9, 12, 14
	X	X	16	p24-qter	D16S539	13	10	10	9	9	9	9	13	9, 9, 10, 13
X	X	X	18	q21.3	D18S51	11	12	11	12	12	12	11	14	11, 12, 12, 14
	X		19	q12-13.1	D19S433	14	14	14	15	14	15	14	15	14, 14, 15
X	X	X	21	q11.2-q21	D21S11	30	33.2	30	33.2	30	33.2	28	30	28, 30, 33.2
	X	X	X	p22.1-22.3	AMEL	X	X	X	Y	X	Y	X	X
	X	X	Y	p11.2	AMEL	X	X	X	Y	X	Y	X	X

Legend

Values possessed by Daughter A and shared by her siblings.

Values possessed by the siblings, but not shared with Daughter A.

Values possessd by C and D only.

Values possessed by one sibling only, so not shared with anyone.

Notes
1. All markers are STR (short tandem repeat) tests on autosomal chromosomes, except for the amelogenin test to determine gender.
2. CODIS = Combined DNA Index System; DNA profiling system for the US Federal Bureau of Investigation (FBI).
3. SGM(+) = Second Generation Multiplex (Plus); DNA profiling system for the UK National Criminal Intelligence DNA Database.

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