Test results for the descendant of Charles's son, Basil, show he has a 66/67 match with the modal haplotype for descendants of the immigrant, Peter I, thus confirming the long-assumed connection.  The descendant of Josiah has tested only 12 markers, and he is a 12/12 match with the modal haplotype of the descendants of Peter I.  However, the results for the two brothers who are descendants of Charles's son, Reason, do not remotely match the rest of these CARRICOs.  The inescapable conclusion is that the two brothers descended from Reason have an NPE, a "non-paternal event" (i.e., hidden adoption or illicit paternity), in their patrilineal line, somewhere between their father and Reason's father.

Fortunately, despite being Haplogroup R-1b1b2, the most common Y-DNA haplogroup in western Europe, these two CARRICO brothers have a unique haplotype, even at 12 markers.  They have only one near match in the FTDNA, Ysearch, or SMGF databases (at least as of Apr 2009), and that is a 62/67 match with a patrilineal descendant of Farrell LITTLETON.

Farrell is believed to have been born in 1740 in London, England, and to have died ca. 1775 in Virginia.  His grandsons later moved from VA into KY, so it would appear that the NPE had to have occurred in KY, which would make Reason CARRICO a son of John LITTLETON I.  This scenario is only weakly plausible because, although both families were in Kentucky at the time (ca. 1791), the LITTLETONs were in Mason County, while Charles was in Washington County, although he was also said to be in Carroll County.  On the other hand, both Carroll and Mason Counties are on the Ohio River, and travel between cities on the river was comparatively easy.

The 62/67 match is not as close as one would expect from a common ancestor just five generations back, so other scenarios are more likely.  One is that the connection is to a different branch of this LITTLETON family, with a common ancestor ten to fifteen generations removed.  In other words, to a different LITTLETON immigrant than Farrell, one whose descendants have yet to be tested.  Given that this paper descendant of Farrell has no match in the LITTLETON project, it may also turn out that he has an NPE in his lineage, too.  These questions can be answered by testing more individuals.  If doing genealogy requires patience — and we all know it does — doing genetic genealogy can require extreme patience.

By testing near kin and cousins, Reason's descendant can at least determine the generation in which the NPE occurred. 

If that descendant matches #89853, then
we can assume that neither has an NPE in 
his line.  If they don't match, then we need to
test the descendant of a different brother to
see who is "odd man out," with the NPE.

Unless John I has some brothers to test, we
won't know whether or not John I has an NPE
until these LITTLETONs start matching some
other line of LITTLETONs — or some other
surname.